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Objective@#To investigate the association between single nucleotide polymorphisms (SNP) in cytokine IL6, IL10 genes and the susceptibility to primary hepatic carcinoma(PHC) of Shunde district in Guangdong Province.@*Methods@#Patients from two hospitals in Shunde District of Foshan City were selected from October 2010 to October 2012. Case group inclusion criteria includedprimary liver cancer; local residents of Shunde or living in Shunde for more than 10 years. The control group inclusion criteria included: patients visited ENT, general surgery and physical examination department in the same hospital during the same period; Local residents or living in Shunde for 10 years and above. The control group was matched 1∶1 by gender, and age (±3 years old) with case group. A total of 306 subjects were collected. Questionnaires were used to investigate the information including demographic characteristics, PHC status survey and so on. The venous blood was collected from each subject to extract DNA, and to detect label SNP site and genotype. Hardy-Weinberg equilibrium was detected in the control group by the goodness-of-fit χ2 test. Multivariate conditional logistic regression was used to estimate the relationship between IL6 (rs1800796), IL10 (rs1800871, rs1800872)genes polymorphisms and susceptibility to PHC.@*Results@#There were 264 males and 42 females both in the case group and the control group, with an average age of (55.84±11.49) and (55.83±11.67) years old respectively (t=0.011, P=0.992). The frequencies of IL6 (rs1800796), IL10 (rs1800871) and IL10 (rs1800872) genotypes in the control group were in accordance with the Hardy-Weinberg equilibrium, which indicated the population was representative (all P values>0.05). Conditional logistic regression analysis showed that compared with the AA genotype and AA+AC genotype of IL10(rs1800872), CC genotype increased the risk of PHC by 2.02 times (OR=3.02, 95%CI:1.21-7.56)and 1.89 times (OR=2.89, 95%CI:1.19-7.04)respectively after the smoking history, eating fish history, drinking history, chronic hepatitis B infection, and family history of liver cancer adjusted. No statistical association was found between SNP in cytokine IL6 (rs1800796) and the susceptibility to PHC (P>0.05).@*Conclusion@#The results indicated that people who carried CC genotype in rs1800872 of IL10 gene have an increasing risk of PHC.
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Objective To explore the carry rate and antimicrobial resistance of Staphylococcus aureus (S.aureus) among primary school students.Methods Nasal swab samples were collected from healthy primary school students in Guangzhou.Antibiotic susceptibility testing was applied to test S.aureus strains.Results The overall carriage rate of S.aureus,methicillin-resistant S.aureus (MRSA) and multi-drug-resistant S.aureus (MDRSA) among 1 012 primary school students were 40.1％,1.2％ and 4.0％,respectively.Most S.aureus isolates were resistant to penicillin,erythromycin and clindamycin.The dominant multidrug resistance patterns of MDRSA isolates were resistant to erythromycin-clindamycin-tetracycline and erythromycin-clindamycin-cefoxitin.Multifactor dimensionality reduction analysis showed that the rate of resistance to cefoxitin,tetracycline and chloromycetin among MDRSA was 104.39 times as much as that of nonMDRSA.Conclusions The carriage rate of S.aureus in healthy primary school students from Guangzhou was high and these isolates showed multidrug resistance.These data provide basis for guiding the rational use of antibiotics.
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<p><b>BACKGROUND</b>Most of the researches about lung cancer have focused on the polymorphisms of single gene rather than the cooperation of the genes. The aim of this study is to investigate the correlation of gene polymorphisms of cytochrome P450 2E1 (CYP2E1) Rsa I and cytochrome P450 1A1 (CYP1A1) independently and in combination with the risk of lung cancer.</p><p><b>METHODS</b>A case-control study which included 91 lung cancer cases and 91 hospital-based controls was carried out, and all of them were the Han nationality in Guangzhou district. Blood samples were collected for detecting CYP2E1 Rsa I and CYP1A1 gene polymorphisms by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP).</p><p><b>RESULTS</b>No significant difference was observed in genotype frequencies of CYP2E1 Rsa I and CYP1A1 Msp I between case and control groups. Odd ratio was 5.48 and 1.51 respectively without significant difference (P > 0.05). Individuals with CYP2E1 Rsa I C1C1 and CYP1A1 m2m2 genotype had 3.00-fold risk of developing lung cancer compared with those who had CYP2E1 Rsa I C1C2 and CYP1A1 m1m1 genotype (P < 0.05, 95% CI 1.03-8.78).</p><p><b>CONCLUSIONS</b>Single polymorphism of CYP2E1 and CYP1A1 doesn't increase the risk of developing lung cancer, but the combination of two genetic polymorphisms significantly increases the risk of developing lung cancer.</p>
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In order to get information on the use of antibiotics in rural areas, 300 hospitalized patients were randomly selected from a rural hospital of Guangdong province and investigated for antibiotic use. The rate of antibiotic use in the hospitalized patients was 86.7 %; the four antibiotics at the top of the list were penicilins, cephalosporins, quinolones and macrolide. The rate of combined use of antibiotics was 68.46%. The culture of bacteria was carried out in only 4.62% of the patients and no antimicrobial susceptibility test was conducted. It is concluded that the antibiotics were not used in rational fashion in the rural hospital.
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Objective: To analyze the interaction between serum selenium level and genetic polymorphism of GSTM1 in lung cancer. Methods: A case-control study was conducted which included 58 cases of lung cancer and 62 controls. The serum selenium level was determined by generation hydrogen atomic fluorospectrometry (GHAFS) and the genotypes of GSTM1 were detected by PCR. Results: The OR of lung cancer, comparing the lower level (